Carboxyethyl-functionalized 3D porous polypyrrole synthesized using a porogen-free method for covalent immobilization of urease

The immobilization of enzymes onto porous supports is a common strategy for obtaining improved stability, fast product separation, enzyme reusability, and, ultimately, lower operating costs. Therefore, the development new with specific surface functionalities that enable covalent attachment significant interest. Herein, stable three-dimensional (3D) materials were synthesized from polypyrrole by simple template approach and used as an support urease. method entails use nanoparticle building blocks, which carboxylic acid-functionalized pyrrole monomer was polymerized, forming 3D structure tunable pore size distribution. Scanning electron microscopy (SEM) images, together static light scattering (SLS), revealed nature materials. properties both immobilized characterized using combination techniques. ability bioconjugated urease to catalyze hydrolysis urea into carbon dioxide ammonia then tested. exhibited good catalytic activity, stability reusability. Overall, these results suggest such chemically accessible surfaces have considerable potential biocatalyst supports.